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Biomedicines
Volume 12
Issue 12
10.3390/biomedicines12122731
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Open AccessArticle
by Xiangguo Che SciProfiles Scilit Preprints.org Google Scholar Hee-June Kim SciProfiles Scilit Preprints.org Google Scholar Xian Jin
Xiangguo Che
, Hee-June Kim
Xian Jin
SciProfiles Scilit Preprints.org Google Scholar
, Joon-Woo Kim
Joon-Woo Kim
SciProfiles Scilit Preprints.org Google Scholar
, Kyeong-Hyeon Park
Kyeong-Hyeon Park
SciProfiles Scilit Preprints.org Google Scholar
, Jeong-Ok Lim
Jeong-Ok Lim
SciProfiles Scilit Preprints.org Google Scholar
Hee-Soo Kyung
Hee-Soo Kyung
SciProfiles Scilit Preprints.org Google Scholar
Chang-Wug Oh
Chang-Wug Oh
SciProfiles Scilit Preprints.org Google Scholar
and Je-Yong Choi
Je-Yong Choi
SciProfiles Scilit Preprints.org Google Scholar
1
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea
2
Department of Orthopedic Surgery, Kyungpook National University Hospital, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea
3
Severance Children’s Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
4
Joint Institute for Regenerative Medicine, Kyungpook National University, Daegu 41940, Republic of Korea
5
Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu 41940, Republic of Korea
*
Authors to whom correspondence should be addressed.
Biomedicines 2024, 12(12), 2731; https://doi.org/10.3390/biomedicines12122731 (registeringDOI)
Submission received: 22 October 2024 / Revised: 21 November 2024 / Accepted: 27 November 2024 / Published: 28 November 2024
(This article belongs to the Special Issue Musculoskeletal Injuries: Clinical, Radiological, and Biochemical Evaluation in Orthopedics)
Abstract
Background: Bone marrow aspiration concentrate (BMAC) has garnered increasing interest due to its potential for healing musculoskeletal injuries. While the iliac crest remains a common harvest site, the aspiration technique’s efficacy in offering the highest yield and prevalence of mesenchymal stem cells (MSCs) is controversial. This study aimed to compare two different techniques of bone marrow aspiration over the anterior iliac crest from a single level versus multiple levels. Methods: Anterior iliac crests were selected in seven adult patients (aged between 31 and 59 years old). Aspiration was achieved using an 11-gauge needle (length: 100 mm; diameter: 2.3 mm) specifically manufactured for bone marrow collection (BD, Becton, Franklin Lakes, NJ, USA) connected to a 10 mL syringe. On one side, 4cc of bone marrow was aspirated at a single level to a depth of 7 cm without changing the needle direction. On the other side, over the same portion of the iliac crest, 1 cc of bone marrow was obtained from multiple levels of different depths during needle retrieval, maintaining a distance of 1 cm and changing the tip direction. The samples were blindly sent to the laboratory without indicating whether they came from an single level or multiple levels. Fluorescence-activated cell sorting (FACS) and osteoblast differentiation were analyzed and compared. Results: In the FACS analysis, the single level resulted in a higher population of MSCs that were positive for CD105, CD73, and CD90 and negative for CD34, compared to the multiple-level method. In the process of osteoblast differentiation, it was observed that MSCs exhibited more advanced features of enhanced osteoblastic abilities in the single-level method rather than the multiple-level method. Conclusions: A single-level aspiration technique at the anterior iliac crest may produce a high-quality bone marrow aspirate. This technique may help obtain specific populations of MSCs with the desired characteristics for use in regenerative therapies for musculoskeletal injuries.
Keywords: bone marrow aspiration concentrate; mesenchymal stem cells; single level; multiple levels; musculoskeletal injuries
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MDPI and ACS Style
Che, X.; Kim, H.-J.; Jin, X.; Kim, J.-W.; Park, K.-H.; Lim, J.-O.; Kyung, H.-S.; Oh, C.-W.; Choi, J.-Y. Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines 2024, 12, 2731. https://doi.org/10.3390/biomedicines12122731
AMA Style
Che X, Kim H-J, Jin X, Kim J-W, Park K-H, Lim J-O, Kyung H-S, Oh C-W, Choi J-Y. Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines. 2024; 12(12):2731. https://doi.org/10.3390/biomedicines12122731
Chicago/Turabian Style
Che, Xiangguo, Hee-June Kim, Xian Jin, Joon-Woo Kim, Kyeong-Hyeon Park, Jeong-Ok Lim, Hee-Soo Kyung, Chang-Wug Oh, and Je-Yong Choi. 2024. "Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration" Biomedicines 12, no. 12: 2731. https://doi.org/10.3390/biomedicines12122731
APA Style
Che, X., Kim, H. -J., Jin, X., Kim, J. -W., Park, K. -H., Lim, J. -O., Kyung, H. -S., Oh, C. -W., & Choi, J. -Y. (2024). Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines, 12(12), 2731. https://doi.org/10.3390/biomedicines12122731
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MDPI and ACS Style
Che, X.; Kim, H.-J.; Jin, X.; Kim, J.-W.; Park, K.-H.; Lim, J.-O.; Kyung, H.-S.; Oh, C.-W.; Choi, J.-Y. Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines 2024, 12, 2731. https://doi.org/10.3390/biomedicines12122731
AMA Style
Che X, Kim H-J, Jin X, Kim J-W, Park K-H, Lim J-O, Kyung H-S, Oh C-W, Choi J-Y. Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines. 2024; 12(12):2731. https://doi.org/10.3390/biomedicines12122731
Chicago/Turabian Style
Che, Xiangguo, Hee-June Kim, Xian Jin, Joon-Woo Kim, Kyeong-Hyeon Park, Jeong-Ok Lim, Hee-Soo Kyung, Chang-Wug Oh, and Je-Yong Choi. 2024. "Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration" Biomedicines 12, no. 12: 2731. https://doi.org/10.3390/biomedicines12122731
APA Style
Che, X., Kim, H. -J., Jin, X., Kim, J. -W., Park, K. -H., Lim, J. -O., Kyung, H. -S., Oh, C. -W., & Choi, J. -Y. (2024). Bone Marrow Stem Cell Population in Single- and Multiple-Level Aspiration. Biomedicines, 12(12), 2731. https://doi.org/10.3390/biomedicines12122731
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
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